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KMID : 0861020150300010025
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Korea Journal of Herbology
2015 Volume.30 No. 1 p.25 ~ p.32
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Effect of Cassiae semen extract on ovalbumin-induced allergic asthma in mice
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Seo Beom-Su
Park Yong-Kee
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Abstract
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Objectives : In this study, we investigated the effect of Cassia obtusifolia Linne (Cassiae Semen; CS) extract on ovalbumin (OVA)-induced allergic asthma in mice.
Methods : CR was extracted with 70% ethanol. For in vitro study, HMC-1, human mast cells were treated with CS extract at 0.2 and 0.5 ㎎/㎖for 1 h, and then stimulated with compound (C) 48/80 for 30 min. Primary spleenocytes were isolated from the spleen of mice, treated with CS extract for 1 h, and then stimulated with ConA for 24 h. For in vivo study, mice were sensitized at day 0, 7 and 14 with 0.2% OVA and then airway challenged using neublizer at day 21, 23, 25, and 27 to induced allergic asthma. CS extract at doses of 100 and 300 mg/kg body weight was orally administered during OVA challenge once per a day. The levels of allergic mediators such as histamine, OVA-specific IgE, IL-4, and IFN-γ were measured in the sera of mice or culture supernatants by EIA and ELISA, respectively. The expression of IL-4 and IFN-γ gene was determined by RT-PCR. The histopathological change of lung tissues was observed with hematoxylin and eosin (H&E) and Periodic acid Schiff (PAS) staining.
Results : The treatment of CS extract in HMC-1 cells significantly inhibited C48/80-induced degranulation, and histamine release. The treatment of CS extract in spleenocytes suppressed the expression of IL-4 and IFN-γ mRNA. The administration of CS extract in OVA-induced asthmatic mice significantly decreased the levels of OVA-specific IgE, and IL-4 in a dose-dependent manner with OVA-control group. In addition, CS extract inhibited the infiltration of inflammatory cells and bronchiolar damage with epithelial thickening in lung tissues of OVA-induced asthma mice, and also mucin accumulation.
Conclusions : These results indicate that CS extract prevents asthmatic damage through regulating the allergic immune response.
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KEYWORD
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Cassia obtusifolia Linne, Cassiae Semen, asthma, ovalbumin, mast cell, histamine, immunoglobulin E, IL-4, IFN-γ
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